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Biotechnol Lett. 2012 Jun;34(6):1123-30. doi: 10.1007/s10529-012-0883-x. Epub 2012 Feb 25.

Improvement of D-lactate productivity in recombinant Escherichia coli by coupling production with growth.

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Center for Bioresource and Bioenergy, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.


Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm and 42°C, achieved a twofold higher productivity of lactic acid compared to aerobic and O(2)-limited two-phase fermentation. The cells in the bioreactor yielded an overall volumetric productivity of 5.5 g/l h and a yield of 86 g lactic acid/100 g glucose which were 66% higher and the same level compared to that of the aerobic and O(2)-limited two-phase fermentation, respectively, using scaled-up conditions optimized from shake-flask experiments. These results have revealed an approach for improving production of fermentative products in E. coli.

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