Format

Send to

Choose Destination
See comment in PubMed Commons below
Genetics. 2012 May;191(1):163-70. doi: 10.1534/genetics.111.137497. Epub 2012 Feb 23.

Tracing the emergence of a novel sex-determining gene in medaka, Oryzias luzonensis.

Author information

1
Institute of Science and Technology, Niigata University, Niigata 950-2181, Japan. f09m004a@mail.cc.niigata-u.ac.jp

Abstract

Three sex-determining (SD) genes, SRY (mammals), Dmy (medaka), and DM-W (Xenopus laevis), have been identified to date in vertebrates. However, how and why a new sex-determining gene appears remains unknown, as do the switching mechanisms of the master sex-determining gene. Here, we used positional cloning to search for the sex-determining gene in Oryzias luzonensis and found that GsdfY (gonadal soma derived growth factor on the Y chromosome) has replaced Dmy as the master sex-determining gene in this species. We found that GsdfY showed high expression specifically in males during sex differentiation. Furthermore, the presence of a genomic fragment that included GsdfY converts XX individuals into fertile XX males. Luciferase assays demonstrated that the upstream sequence of GsdfY contributes to the male-specific high expression. Gsdf is downstream of Dmy in the sex-determining cascade of O. latipes, suggesting that emergence of the Dmy-independent Gsdf allele led to the appearance of this novel sex-determining gene in O. luzonensis.

PMID:
22367037
PMCID:
PMC3338257
DOI:
10.1534/genetics.111.137497
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center