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Bioorg Med Chem. 2012 Apr 1;20(7):2416-8. doi: 10.1016/j.bmc.2012.01.044. Epub 2012 Feb 4.

The synthesis of 2'-methylseleno adenosine and guanosine 5'-triphosphates.

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1
Institute of Organic Chemistry and Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck, Austria.

Abstract

Modified nucleoside triphosphates (NTPs) represent powerful building blocks to generate nucleic acids with novel properties by enzymatic synthesis. We have recently demonstrated the access to 2'-SeCH(3)-uridine and 2'-SeCH(3)-cytidine derivatized RNAs for applications in RNA crystallography, using the corresponding nucleoside triphosphates and distinct mutants of T7 RNA polymerase. In the present note, we introduce the chemical synthesis of the novel 2'-methylseleno-2'-deoxyadenosine and -guanosine 5'-triphosphates (2'-SeCH(3)-ATP and 2'-SeCH(3)-GTP) that represent further candidates for the enzymatic RNA synthesis with engineered RNA polymerases.

PMID:
22364745
PMCID:
PMC3334826
DOI:
10.1016/j.bmc.2012.01.044
[Indexed for MEDLINE]
Free PMC Article
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