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Planta Med. 1990 Aug;56(4):364-7.

Growth, terpenoid production and antibacterial activity of an in vitro culture of the liverwort Fossombronia pusilla.

Author information

1
Pharmakognosie und Analytische Phytochemie, Universit├Ąt des Saarlandes, Federal Republic of Germany.

Abstract

In vitro cultures of the liverwort, Fossombronia pusilla, have been ititiated from spores. They grew well on Gamborg B5 medium supplemented with 2% saccharose and 12.5 micrograms/l vitamin B12. Undifferentiated cultures were obtained on Knop mineral solution with 4% glucose. The cultures produced the same constituents as collected material. Perrottetianal A and B, known substances from liverworts were isolated and characterized by spectroscopic methods as well as alpha-(-)-santonin. A hitherto unknown diterpene dialdehyde-8-hydroxy-9-hydroperrottetianal A--could also be characterized. Furthermore, 7 terpenes were identified by GC/MS. A petrol ether extract and the isolated terpenes exerted antibacterial activity.

PMID:
2236288
DOI:
10.1055/s-2006-960983
[Indexed for MEDLINE]

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