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Biotechnol Bioeng. 2012 Aug;109(8):1929-36. doi: 10.1002/bit.24458. Epub 2012 Feb 22.

Consolidated bioprocessing (CBP) of AFEX™-pretreated corn stover for ethanol production using Clostridium phytofermentans at a high solids loading.

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Biomass Conversion Research Laboratory, Department of Chemical Engineering and Materials Science, Michigan State University, Lansing, Michigan 48910, USA.


Consolidated bioprocessing (CBP) using Clostridium phytofermentans (ATCC 700394) on ammonia fiber expansion (AFEX™)-treated corn stover (AFEX™-CS) at a low solids loading showed promising results [Jin et al. (2011) Biotechnol Bioeng 108(6): 1290-1297]. However, industrial relevant process requires high solids loading. Therefore, we studied high solids loading CBP performance on AFEX™-CS. The factors potentially affecting the performance including solids loading, CBP products acetate and ethanol, and degradation products resulting from pretreatment were investigated. At 4% (w/w) glucan loading, C. phytofermentans performed well on AFEX™-CS with no nutrients supplementation and reached similar sugar conversions as a fermentation with nutrients supplementation. A glucan conversion of 48.9% and a xylan conversion of 77.9% were achieved after 264 h with 7.0 g/L ethanol and 8.8 g/L acetate produced. Relatively high concentrations of acetate produced at high solids loading was found to be the major factor limiting the CBP performance. Degradation products in AFEX™-CS helped enhance ethanol production.

[Indexed for MEDLINE]

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