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Biotechnol Bioeng. 2012 Aug;109(8):1929-36. doi: 10.1002/bit.24458. Epub 2012 Feb 22.

Consolidated bioprocessing (CBP) of AFEX™-pretreated corn stover for ethanol production using Clostridium phytofermentans at a high solids loading.

Author information

1
Biomass Conversion Research Laboratory, Department of Chemical Engineering and Materials Science, Michigan State University, Lansing, Michigan 48910, USA. jinmingj@egr.msu.edu

Abstract

Consolidated bioprocessing (CBP) using Clostridium phytofermentans (ATCC 700394) on ammonia fiber expansion (AFEX™)-treated corn stover (AFEX™-CS) at a low solids loading showed promising results [Jin et al. (2011) Biotechnol Bioeng 108(6): 1290-1297]. However, industrial relevant process requires high solids loading. Therefore, we studied high solids loading CBP performance on AFEX™-CS. The factors potentially affecting the performance including solids loading, CBP products acetate and ethanol, and degradation products resulting from pretreatment were investigated. At 4% (w/w) glucan loading, C. phytofermentans performed well on AFEX™-CS with no nutrients supplementation and reached similar sugar conversions as a fermentation with nutrients supplementation. A glucan conversion of 48.9% and a xylan conversion of 77.9% were achieved after 264 h with 7.0 g/L ethanol and 8.8 g/L acetate produced. Relatively high concentrations of acetate produced at high solids loading was found to be the major factor limiting the CBP performance. Degradation products in AFEX™-CS helped enhance ethanol production.

PMID:
22359098
DOI:
10.1002/bit.24458
[Indexed for MEDLINE]

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