Abstract
Previous studies in Caenorhabditis elegans showed that RPM-1 (Regulator of Presynaptic Morphology-1) regulates axon termination and synapse formation. To understand the mechanism of how rpm-1 functions, we have used mass spectrometry to identify RPM-1 binding proteins, and have identified RAE-1 (RNA Export protein-1) as an evolutionarily conserved binding partner. We define a RAE-1 binding region in RPM-1, and show that this binding interaction is conserved and also occurs between Rae1 and the human ortholog of RPM-1 called Pam (protein associated with Myc). rae-1 loss of function causes similar axon and synapse defects, and synergizes genetically with two other RPM-1 binding proteins, GLO-4 and FSN-1. Further, we show that RAE-1 colocalizes with RPM-1 in neurons, and that rae-1 functions downstream of rpm-1. These studies establish a novel postmitotic function for rae-1 in neuronal development.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / metabolism
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Amino Acid Motifs / genetics
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Amino Acid Sequence / genetics
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Animals
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Animals, Genetically Modified
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Axons / physiology*
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Caenorhabditis elegans
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism
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F-Box Proteins / genetics
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F-Box Proteins / metabolism
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Gene Expression Regulation / genetics
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Guanine Nucleotide Exchange Factors / genetics
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Guanine Nucleotide Exchange Factors / metabolism
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Humans
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Immunoprecipitation
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Luminescent Proteins / genetics
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Mass Spectrometry
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Mechanoreceptors / cytology*
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Microscopy, Confocal
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Molecular Sequence Data
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Mutation / genetics
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Nuclear Matrix-Associated Proteins / deficiency
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Nuclear Matrix-Associated Proteins / genetics
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Nuclear Matrix-Associated Proteins / metabolism*
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Nucleocytoplasmic Transport Proteins / deficiency
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Nucleocytoplasmic Transport Proteins / genetics
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Nucleocytoplasmic Transport Proteins / metabolism*
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Protein Binding / genetics
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Signal Transduction / genetics
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Synapses / genetics
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Synapses / physiology*
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Ubiquitin-Protein Ligases / genetics
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Ubiquitin-Protein Ligases / metabolism
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rab GTP-Binding Proteins / genetics
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rab GTP-Binding Proteins / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Caenorhabditis elegans Proteins
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F-Box Proteins
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FSN-1 protein, C elegans
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Guanine Nucleotide Exchange Factors
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Luminescent Proteins
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Nuclear Matrix-Associated Proteins
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Nucleocytoplasmic Transport Proteins
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RAE1 protein, human
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RPM-1 protein, C elegans
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MYCBP2 protein, human
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Ubiquitin-Protein Ligases
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Glo-1 protein, C elegans
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rab GTP-Binding Proteins