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J Infect Dis. 2012 Apr 1;205(7):1095-9. doi: 10.1093/infdis/jis032. Epub 2012 Feb 22.

CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA.

Author information

1
Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287, USA.

Abstract

We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

PMID:
22357696
PMCID:
PMC3295606
DOI:
10.1093/infdis/jis032
[Indexed for MEDLINE]
Free PMC Article

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