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J Clin Microbiol. 2012 May;50(5):1764-8. doi: 10.1128/JCM.00394-12. Epub 2012 Feb 22.

Development of a transcription-reverse transcription concerted reaction method for specific detection of human enterovirus 71 from clinical specimens.

Author information

1
Tokyo Research Center, Tosoh Corp., Hayakawa, Ayase City, Kanagawa, Japan.

Abstract

A transcription-reverse transcription (RT) concerted reaction (TRCR) method was developed for rapid and specific detection of EV71 from clinical specimens. This method was validated with EV71 strains from all of the known genotypes (genotypes A, B1 to B5, and C1 to C5), with detection limits of 10 to 10(3) copies, and was useful for identification of EV71 from throat swabs of patients with hand, foot, and mouth disease (HFMD).

PMID:
22357494
PMCID:
PMC3347152
DOI:
10.1128/JCM.00394-12
[Indexed for MEDLINE]
Free PMC Article

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