Format

Send to

Choose Destination
J Vet Intern Med. 2012 Mar-Apr;26(2):282-93. doi: 10.1111/j.1939-1676.2012.00891.x. Epub 2012 Feb 22.

Urinary biomarkers of renal disease in dogs with X-linked hereditary nephropathy.

Author information

1
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77483-4467, USA. mnabity@cvm.tamu.edu

Abstract

BACKGROUND:

Sensitive and specific biomarkers for early tubulointerstitial injury are lacking.

HYPOTHESIS:

The excretion of certain urinary proteins will correlate with the state of renal injury in dogs with chronic kidney disease.

ANIMALS:

Twenty-five male colony dogs affected with X-linked hereditary nephropathy (XLHN) and 19 unaffected male littermates were evaluated.

METHODS:

Retrospective analysis of urine samples collected every 2-4 weeks was performed. Urine proteins evaluated were retinol binding protein (uRBP/c), β2-microglobulin (uB2M), N-acetyl-β-D-glucosaminidase (uNAG/c), neutrophil gelatinase-associated lipocalin (uNGAL/c), and immunoglobulin G (uIgG/c). Results were correlated with serum creatinine concentration (sCr), glomerular filtration rate (GFR), urine protein : creatinine ratio, and histopathologic analysis of serial renal biopsies. Analytical validation was performed for all assays; uNAG stability was evaluated.

RESULTS:

All urinary biomarkers distinguished affected dogs from unaffected dogs early in their disease process, increasing during early and midstages of disease. uRBP/c correlated most strongly with conventional measures of disease severity, including increasing sCr (r = 0.89), decreasing GFR (r = -0.77), and interstitial fibrosis (r = 0.80), P < .001. However, multivariate analysis revealed age, sCr, uIgG/c, and uB2M, but not uRBP/c, as significant independent predictors of GFR (P < .05).

CONCLUSIONS AND CLINICAL IMPORTANCE:

All urinary biomarkers were elevated before sCr increased, but typically after proteinuria developed in dogs with progressive glomerular disease because of XLHN. uRBP/c measurement might be promising as a noninvasive tool for diagnosis and monitoring of tubular injury and dysfunction in dogs.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center