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Sci Rep. 2011;1:153. doi: 10.1038/srep00153. Epub 2011 Nov 11.

The elementary events underlying force generation in neuronal lamellipodia.

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1
Neurobiology Sector, International School for Advanced Studies (SISSA), IT-34136 Trieste, Italy.

Abstract

We have used optical tweezers to identify the elementary events underlying force generation in neuronal lamellipodia. When an optically trapped bead seals on the lamellipodium membrane, Brownian fluctuations decrease revealing the underlying elementary events. The distribution of bead velocities has long tails with frequent large positive and negative values associated to forward and backward jumps occurring in 0.1-0.2 ms with varying amplitudes up to 20 nm. Jump frequency and amplitude are reduced when actin turnover is slowed down by the addition of 25 nM Jasplakinolide. When myosin II is inhibited by the addition of 20 μM Blebbistatin, jump frequency is reduced but to a lesser extent than by Jasplainolide. These jumps constitute the elementary events underlying force generation.

PMID:
22355669
PMCID:
PMC3240973
DOI:
10.1038/srep00153
[Indexed for MEDLINE]
Free PMC Article
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