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Sci Rep. 2011;1:51. doi: 10.1038/srep00051. Epub 2011 Aug 1.

Cross-reactivity and expansion of dengue-specific T cells during acute primary and secondary infections in humans.

Author information

1
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01655, USA.

Abstract

Serotype-cross-reactive memory T cells responding to secondary dengue virus (DENV) infection are thought to contribute to disease. However, epitope-specific T cell responses have not been thoroughly compared between subjects with primary versus secondary DENV infection. We studied CD8(+) T cells specific for the HLA-A*1101-restricted NS3(133) epitope in a cohort of A11(+) DENV-infected patients throughout acute illness and convalescence. We compared the expansion, serotype-cross-reactivity, and activation of these cells in PBMC from patients experiencing primary or secondary infection and mild or severe disease by flow cytometry. Our results show expansion and activation of DENV-specific CD8(+) T cells during acute infection, which are predominantly serotype-cross-reactive regardless of DENV infection history. These data confirm marked T cell activation and serotype-cross-reactivity during the febrile phase of dengue; however, A11-NS3(133)-specific responses did not correlate with prior antigenic exposure or current disease severity.

PMID:
22355570
PMCID:
PMC3216538
DOI:
10.1038/srep00051
[Indexed for MEDLINE]
Free PMC Article

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