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J Biomed Opt. 2012 Jan;17(1):015003. doi: 10.1117/1.JBO.17.1.015003.

Investigating photoexcitation-induced mitochondrial damage by chemotherapeutic corroles using multimode optical imaging.

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1
Minimally Invasive Surgical Technologies Institute, Cedars-Sinai Medical Center, 8700 Beverly Boulevard D6061, Los Angeles, California 90048, USA.

Abstract

We recently reported that a targeted, brightly fluorescent gallium corrole (HerGa) is highly effective for breast tumor detection and treatment. Unlike structurally similar porphryins, HerGa exhibits tumor-targeted toxicity without the need for photoexcitation. We have now examined whether photoexcitation further modulates HerGa toxicity, using multimode optical imaging of live cells, including two-photon excited fluorescence, differential interference contrast (DIC), spectral, and lifetime imaging. Using two-photon excited fluorescence imaging, we observed that light at specific wavelengths augments the HerGa-mediated mitochondrial membrane potential disruption of breast cancer cells in situ. In addition, DIC, spectral, and fluorescence lifetime imaging enabled us to both validate cell damage by HerGa photoexcitation and investigate HerGa internalization, thus allowing optimization of light dose and timing. Our demonstration of HerGa phototoxicity opens the way for development of new methods of cancer intervention using tumor-targeted corroles.

PMID:
22352647
PMCID:
PMC3380813
DOI:
10.1117/1.JBO.17.1.015003
[Indexed for MEDLINE]
Free PMC Article
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