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Methods Mol Biol. 2012;832:185-96. doi: 10.1007/978-1-61779-474-2_13.

Using linkage-specific monoclonal antibodies to analyze cellular ubiquitylation.

Author information

1
Physiological Chemistry Department, Genentech, Inc., South San Francisco, CA, USA.

Abstract

Antibodies that specifically recognize polyubiquitin chains containing ubiquitins linked at a particular lysine residue are powerful tools for interrogating endogenous protein modifications. Here, we describe protocols for revealing K11-, K48-, and K63-linked polyubiquitin chains by western blotting, immunoprecipitation, or immunostaining.

PMID:
22350886
DOI:
10.1007/978-1-61779-474-2_13
[Indexed for MEDLINE]

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