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Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):300-9. doi: 10.1107/S0907444912001862. Epub 2012 Feb 14.

High-pressure-induced water penetration into 3-isopropylmalate dehydrogenase.

Author information

1
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Japan.

Abstract

Hydrostatic pressure induces structural changes in proteins, including denaturation, the mechanism of which has been attributed to water penetration into the protein interior. In this study, structures of 3-isopropylmalate dehydrogenase (IPMDH) from Shewanella oneidensis MR-1 were determined at about 2 Å resolution under pressures ranging from 0.1 to 650 MPa using a diamond anvil cell (DAC). Although most of the protein cavities are monotonically compressed as the pressure increases, the volume of one particular cavity at the dimer interface increases at pressures over 340 MPa. In parallel with this volume increase, water penetration into the cavity could be observed at pressures over 410 MPa. In addition, the generation of a new cleft on the molecular surface accompanied by water penetration could also be observed at pressures over 580 MPa. These water-penetration phenomena are considered to be initial steps in the pressure-denaturation process of IPMDH.

PMID:
22349232
PMCID:
PMC3282623
DOI:
10.1107/S0907444912001862
[Indexed for MEDLINE]
Free PMC Article

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