Send to

Choose Destination
Cell Div. 2012 Feb 20;7:5. doi: 10.1186/1747-1028-7-5.

Kinetics of DNA methylation inheritance by the Dnmt1-including complexes during the cell cycle.

Author information

Institut de Recherche Thérapeutique de l'Université de Nantes, INSERM U892, Centre de Recherche en Cancérologie Nantes-Angers, Equipe Apoptose et Progression Tumorale, 8 quai moncousu, BP7021, 44007 Nantes, France.



The clonal transmission of lineage-specific DNA methylation patterns in a mammalian genome during the cellular division is a crucial biological process controlled by the DNA methyltransferase Dnmt1, mainly. To investigate possible dynamic mechanisms of DNA methylation inheritance during the cell cycle, we used a Proximity Ligation In Situ Assay (P-LISA) to analyze the kinetic of formation and DNA recruitment of Dnmt1-including complexes.


P-LISA, sequential chromatin immunoprecipitation and quantitative methylation specific PCR revealed that the Dnmt1/PCNA/UHRF1-including complexes are mainly formed and recruited on DNA during the S-phase of cell cycle, while the formation and the DNA recruitment of several Dnmt1/transcription factors-including complexes are not S-phase dependent but are G0/G1 and/or G2/M phases dependent.


Our data confirm that DNA methylation inheritance occurs in S-phase, and demonstrate that DNA methylation inheritance can also occur in G0/G1 and G2/M phases of the cell cycle.

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center