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Neurosci Res. 2012 May;73(1):85-91. doi: 10.1016/j.neures.2012.02.003. Epub 2012 Feb 17.

A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination.

Author information

1
Institute for Virus Research, Kyoto University, Kyoto, Japan. iimayosh@virus.kyoto-u.ac.jp

Abstract

Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression.

PMID:
22343123
DOI:
10.1016/j.neures.2012.02.003
[Indexed for MEDLINE]

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