Format

Send to

Choose Destination
Enferm Infecc Microbiol Clin. 2013 Feb;31(2):97-9. doi: 10.1016/j.eimc.2012.01.003. Epub 2012 Feb 15.

Algorithm proposal based on the C. Diff Quik Chek Complete ICT device for detecting Clostridium difficile infection.

Author information

1
Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain. morellana.hdoc@salud.madrid.org

Abstract

OBJECTIVE:

To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C. difficile infection (CDI) based on this test.

METHODS:

We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the reference.

RESULTS:

This test enabled us to obtain a conclusive result in <30min in 93.8% of the samples. Among the discrepant results (GDH (+)/Toxin A/B (-)), 41.7% (25/60) were found to be toxigenic C. difficile by toxigenic culture.

CONCLUSION:

This test has a high sensitivity and specificity for the diagnosis of CDI.

PMID:
22341752
DOI:
10.1016/j.eimc.2012.01.003
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center