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J Pediatr Nurs. 2012 Apr;27(2):163-7. doi: 10.1016/j.pedn.2010.11.003. Epub 2011 Jan 31.

Clinical guidance on the use of live attenuated influenza vaccine after inadvertent freezing and warming.

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MedImmune, Gaithersburg, MD, USA.


The immediate and long-term impact of temperature deviations that may occur in clinical practice on live attenuated influenza vaccine (LAIV) potency was examined in four distinct studies that exposed vaccine to freeze/thaw cycles, warming, and heating conditions. No significant loss of vaccine potency was observed after three freeze/thaw cycles, warming of vaccine to 15°C (59°F) for 72 hours or less, exposure to room temperature (25°C/77°F) for 12 hours or less, or after heating to 37°C (99°F) for 6 hours or less. The results of these studies demonstrate that LAIV potency can potentially be maintained after exposure to temperature deviations. If a particular annual formulation of LAIV is exposed to temperatures outside of the recommended storage range, practitioners should contact the manufacturer for guidance regarding proper vaccine handling.

[Indexed for MEDLINE]

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