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Allergy. 2012 May;67(5):601-8. doi: 10.1111/j.1398-9995.2012.02792.x. Epub 2012 Feb 16.

Basophils are not the key antigen-presenting cells in allergic patients.

Author information

1
Department of Otorhinolaryngology, Medical University of Vienna, AKH, Vienna, Austria. julia.eckl-dorna@meduniwien.ac.at

Abstract

BACKGROUND:

Recent data obtained in mouse models have initiated a controversy whether basophils are the key antigen-presenting cells (APCs) in allergy. Here, we investigate whether basophils are of importance for the presentation of allergen and the induction of T cell proliferation in allergic patients.

METHODS:

T cells, basophils, and APCs depleted of basophils were purified from allergic patients. Co-culture systems based on purified major allergens were established to study allergen-specific T cell responses using proliferation assays.

RESULTS:

Only co-cultures of T cells with APCs depleted of basophils but not with basophils proliferated in response to allergen. Even addition of IL-3 to T cell-basophil co-cultures failed to induce allergen-specific T cell proliferation.

CONCLUSIONS:

Our data demonstrate by classical in vitro proliferation assays that basophils are not key antigen-presenting cells that promote T cell proliferation in secondary immune responses to allergen in allergic patients.

PMID:
22335568
PMCID:
PMC4601523
DOI:
10.1111/j.1398-9995.2012.02792.x
[Indexed for MEDLINE]
Free PMC Article

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