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J Am Chem Soc. 2012 Mar 7;134(9):3942-5. doi: 10.1021/ja209933r. Epub 2012 Feb 22.

Destabilizing domains derived from the human estrogen receptor.

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  • 1Department of Chemical and Systems Biology, Stanford University, Stanford, California 94305, USA.

Abstract

Methods to rapidly and reversibly perturb the functions of specific proteins are desirable tools for studies of complex biological processes. We have demonstrated an experimental strategy to regulate the intracellular concentration of any protein of interest by using an engineered destabilizing protein domain and a cell-permeable small molecule. Destabilizing domains have general utility to confer instability to a wide range of proteins including integral transmembrane proteins. This study reports a destabilizing domain system based on the ligand binding domain of the estrogen receptor that can be regulated by one of two synthetic ligands, CMP8 or 4-hydroxytamoxifen.

PMID:
22332638
PMCID:
PMC3296833
DOI:
10.1021/ja209933r
[PubMed - indexed for MEDLINE]
Free PMC Article
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