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Opt Express. 2012 Jan 30;20(3):3129-43. doi: 10.1364/OE.20.003129.

Maskless imaging of dense samples using pixel super-resolution based multi-height lensfree on-chip microscopy.

Author information

1
Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.

Abstract

Lensfree in-line holographic microscopy offers sub-micron resolution over a large field-of-view (e.g., ~24 mm2) with a cost-effective and compact design suitable for field use. However, it is limited to relatively low-density samples. To mitigate this limitation, we demonstrate an on-chip imaging approach based on pixel super-resolution and phase recovery, which iterates among multiple lensfree intensity measurements, each having a slightly different sample-to-sensor distance. By digitally aligning and registering these lensfree intensity measurements, phase and amplitude images of dense and connected specimens can be iteratively reconstructed over a large field-of-view of ~24 mm2 without the use of any spatial masks. We demonstrate the success of this multi-height in-line holographic approach by imaging dense Papanicolaou smears (i.e., Pap smears) and blood samples.

PMID:
22330550
PMCID:
PMC3364049
DOI:
10.1364/OE.20.003129
[Indexed for MEDLINE]
Free PMC Article

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