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Methods Mol Biol. 2012;852:235-47. doi: 10.1007/978-1-61779-564-0_18.

Design-A-Gene with GeneDesign.

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1
High Throughput Biology Center, Johns Hopkins University School of Public Health, Baltimore, MD, USA.

Abstract

The manual design of synthetic genes is a tedious and error-prone process-even for very short genes-and it becomes completely infeasible when multiple synthetic genes are needed. GeneDesign is a set of modules that automate batch nucleotide manipulation. Here, we explain the installation, configuration, and use of GeneDesign as part of a synthetic design workflow.

PMID:
22328438
DOI:
10.1007/978-1-61779-564-0_18
[Indexed for MEDLINE]
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