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Nat Methods. 2012 Feb 12;9(3):266-9. doi: 10.1038/nmeth.1892.

Spatiotemporal control of gene expression by a light-switchable transgene system.

Author information

1
Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China.

Abstract

We developed a light-switchable transgene system based on a synthetic, genetically encoded light-switchable transactivator. The transactivator binds promoters upon blue-light exposure and rapidly initiates transcription of target transgenes in mammalian cells and in mice. This transgene system provides a robust and convenient way to spatiotemporally control gene expression and can be used to manipulate many biological processes in living systems with minimal perturbation.

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PMID:
22327833
DOI:
10.1038/nmeth.1892
[Indexed for MEDLINE]

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