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Nat Chem Biol. 2012 Feb 12;8(3):235-7. doi: 10.1038/nchembio.779.

Using transcriptome sequencing to identify mechanisms of drug action and resistance.

Author information

1
Laboratory of Chemistry and Cell Biology, The Rockefeller University, New York, New York, USA.

Abstract

Determining mechanisms of drug action in human cells remains a major challenge. Here we describe an approach in which multiple-drug-resistant clones are isolated and transcriptome sequencing is used to find mutations in each clone. Further analysis of mutations common to more than one clone can identify a drug's physiological target and indirect resistance mechanisms, as indicated by our proof-of-concept studies of the cytotoxic anticancer drugs BI 2536 and bortezomib.

PMID:
22327403
PMCID:
PMC3281560
DOI:
10.1038/nchembio.779
[Indexed for MEDLINE]
Free PMC Article

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