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PLoS One. 2012;7(2):e30733. doi: 10.1371/journal.pone.0030733. Epub 2012 Feb 1.

Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types.

Author information

1
Department of Biochemistry, Stanford University School of Medicine, Stanford, California, United States of America.

Abstract

Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.

PMID:
22319583
PMCID:
PMC3270023
DOI:
10.1371/journal.pone.0030733
[Indexed for MEDLINE]
Free PMC Article

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