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Cytometry A. 2012 Apr;81(4):281-3. doi: 10.1002/cyto.a.22024. Epub 2012 Feb 8.

OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Author information

1
Division of Pulmonary and Critical Care Medicine, Duke University Medical Center, Durham, North Carolina, USA. david.murdoch@duke.edu

Abstract

This panel was optimized for the enumeration and phenotypic characterization of T regulatory cells (Tregs) within the CD4⁺ T-cell pool using human peripheral blood mononuclear cells (PBMC) using intranuclear and intracellular staining methods. The panel was optimized for HIV⁺ clinical trial specimens through the use of HIV-infected and normal donor PBMC. Because the panel is to be used in the context of testing cryopreserved PBMC obtained from multiple sites participating in clinical trials, it was essential to develop an assay that performed well using cryopreserved PBMC. Other tissue types have not been tested.

PMID:
22319016
PMCID:
PMC3352600
DOI:
10.1002/cyto.a.22024
[Indexed for MEDLINE]
Free PMC Article
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