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Lab Chip. 2012 Jun 21;12(12):2146-55. doi: 10.1039/c2lc21147e. Epub 2012 Feb 9.

Droplet microfluidics for high-throughput biological assays.

Author information

1
Department of Physics and School of Engineering and Applied Sciences, Harvard University, Cambridge, USA.

Abstract

Droplet microfluidics offers significant advantages for performing high-throughput screens and sensitive assays. Droplets allow sample volumes to be significantly reduced, leading to concomitant reductions in cost. Manipulation and measurement at kilohertz speeds enable up to 10(8) samples to be screened in one day. Compartmentalization in droplets increases assay sensitivity by increasing the effective concentration of rare species and decreasing the time required to reach detection thresholds. Droplet microfluidics combines these powerful features to enable currently inaccessible high-throughput screening applications, including single-cell and single-molecule assays.

PMID:
22318506
DOI:
10.1039/c2lc21147e
[Indexed for MEDLINE]

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