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Methods Mol Biol. 2012;854:287-97. doi: 10.1007/978-1-61779-573-2_20.

Method for protein subfractionation of cardiovascular tissues before DIGE analysis.

Author information

1
Cardiovascular Division, King's College London, London, UK.

Abstract

Difference gel electrophoresis (DIGE) (Electrophoresis 18, 2071-2077, 1997, 1) is widely used in cardiovascular research. However, the dynamic range limitations stemming from contaminating plasma proteins and highly abundant extracellular matrix components can make cardiovascular tissues difficult to analyze. Here we describe a novel methodology for biochemical subfractionation of cardiovascular tissues before DIGE analysis.

PMID:
22311768
DOI:
10.1007/978-1-61779-573-2_20
[Indexed for MEDLINE]

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