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Microbiol Immunol. 2012 Feb;56(2):139-43. doi: 10.1111/j.1348-0421.2012.00411.x.

Acute respiratory infections due to enterovirus 68 in Yamagata, Japan between 2005 and 2010.

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  • 1Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata, Japan.

Abstract

To clarify the epidemiology of enterovirus 68 (EV68), which is one of the most rarely identified enteroviruses, virus isolation and molecular screening using RT-PCR was performed on 6307 respiratory specimens collected at pediatric clinics in Yamagata, Japan between 2005 and 2010. In the years 2005-2009, 10, 1, 2, 0, and 2 (40) EV68-positive cases, respectively, were identified by RT-PCR. In 2010, 40 cases were identified altogether: 2 by isolation only, 26 by RT-PCR only, and 12 by both isolation and RT-PCR. Phylogenetic analysis indicated that plural genetically distinct clusters co-circulated. These results suggest that that difficulty in EV68 isolation leads to an underestimation of the prevalence of EV68 infections.

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