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Nat Methods. 2012 Feb 5;9(3):270-2. doi: 10.1038/nmeth.1871.

Library-free methylation sequencing with bisulfite padlock probes.

Author information

1
Department of Bioengineering, University of California at San Diego, La Jolla, California, USA.

Abstract

Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.

PMID:
22306810
PMCID:
PMC3461232
DOI:
10.1038/nmeth.1871
[Indexed for MEDLINE]
Free PMC Article

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