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Neuroimage. 2012 Apr 2;60(2):933-9. doi: 10.1016/j.neuroimage.2012.01.100. Epub 2012 Jan 28.

Preparation of fixed mouse brains for MRI.

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Mouse Imaging Centre, The Hospital for Sick Children, Toronto, Ontario, Canada.


In fixed mouse brain magnetic resonance images, a high prevalence of fixation artifacts have been observed. Of more than 1700 images of fixed brains acquired at our laboratory, fixation artifacts were present in approximately 30%. In this study, two of these artifacts are described and their causes are identified. A hyperintense rim around the brain is observed when using perfusates reconstituted from powder and delivered at a high flow rate. It is proposed that these perfusion conditions cause blockage of the capillary beds and an increase in pressure that ruptures the vessels, resulting in a blister of liquid below the dura mater. Secondly, gray-white matter contrast inversion is observed when too short a fixation time or too low a concentration of fixative is used, resulting in inadequate fixation. The deleterious consequences of these artifacts for quantitative data analysis are discussed, and precautions for their prevention are provided.

[Indexed for MEDLINE]

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