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Anal Chim Acta. 2012 Mar 9;718:42-6. doi: 10.1016/j.aca.2011.12.057. Epub 2012 Jan 2.

Determination of oxytetracycline in milk samples by polymer inclusion membrane separation coupled to high performance liquid chromatography.

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  • 1Universidad Autónoma del Estado de Hidalgo, Área Académica de Química, Hidalgo, Mexico.


The determination of oxytetracycline in milk samples using a polymer inclusion membrane concept with high performance liquid chromatography (HPLC) was studied. The membranes developed are composed by cellulose acetate as polymer base, Cyanex 923 as carrier and o-nitrophenyl octyl ether as plasticizer. In the optimal conditions, the method exhibits good linearity in the range 0.03-0.20 mg L(-1) with a limit of detection and quantification of 8.2 and 27.3 μg L(-1) respectively. The method was successfully applied to the analysis of milk samples with high selectivity.

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