Based on the remote sensing data and with the help of geographic information system, an integrated assessment was conducted on the eco-environmental vulnerability of Pearl River Delta in 2004-2008. Spatial principal component analysis was used to generate the evaluation indicators, and analytic hierarchy process (AHP) was applied to determine the weights of the evaluation factors. The reasons causing the vulnerability of the eco- environment in Pearl River Delta were discussed. In the study area, its middle part was the most vulnerable region, occupying 34.0% of the total, eastern part was the moderately vulnerable region, accounting for 25.5%, and western part was the lightly and slightly vulnerable areas, accounting for 28.7 and 11.8%, respectively. Totally, the moderately and lightly vulnerable areas occupied 54.2%, indicating that a majority of the Delta was under moderate and light vulnerability. The natural factors affecting the eco-environmental vulnerability of the Delta were altitude, heavy rain days, water and soil erosion rate, flooded infield rate, normalized difference vegetation index (ND VI) and landscape diversity index, whereas the human factors were population density, waste discharge per unit area, exhaust emission per unit area, land use change, chemical fertilization intensity, pesticide application intensity, amount of motor vehicles possessed by ten thousands people, and index of environmental protection investment. The main characteristics of the extremely and heavily vulnerable regions were low altitude, high frequency of flood disaster, large flooded infield, serious vegetation degradation, high pollution level and low environment protection investment index.