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J Immunol Methods. 1990 Oct 19;133(2):279-84.

An enzyme-linked immunosorbent assay for the measurement of human interleukin-6.

Author information

1
Basic Research Laboratories, Toray Industries, Inc., Kanagawa, Japan.

Abstract

An enzyme-linked immunosorbent assay has been developed to measure human interleukin-6. The assay, based on the avidin-biotin amplified two-step sandwich method, is quick (requiring 4.5 h), sensitive (detecting 9.5 pg/ml) and satisfactory in reproducibility and specificity. It shows good correspondence with the results of bioassays, and it is not affected by serum and plasma components. These results indicate that this ELISA is suitable for application to clinical samples, which is a major advantage over the widely used bioassays.

PMID:
2230142
DOI:
10.1016/0022-1759(90)90369-7
[Indexed for MEDLINE]

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