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Syst Appl Microbiol. 2012 Dec;35(8):513-7. doi: 10.1016/j.syapm.2011.12.001. Epub 2012 Feb 1.

Fluorescence in situ hybridization (FISH) detection of nitrite reductase transcripts (nirS mRNA) in Pseudomonas stutzeri biofilms relative to a microscale oxygen gradient.

Author information

1
Department of Bioscience, Microbiology, Aarhus University, Ny Munkegade 114, DK-8000 Aarhus C, Denmark.

Abstract

Microsensor measurements of oxygen were combined with mRNA-targeted fluorescence in situ hybridization (FISH) to relate the expression of nitrite reductase (nirS) to oxygen concentrations in artificial biofilms of the denitrifier Pseudomonas stutzeri. A distinct zone of nirS transcript-containing cells was detected at the oxic-anoxic transition zone, below an oxygen threshold concentration of 0.7-2.5μM, depending on incubation conditions. Although not a routine technique yet, the possibility of coupling microsensor and mRNA-targeted FISH analyses described here opens for studies addressing microenvironment, identity, and actual activity of microbes in stratified environments at single cell resolution.

PMID:
22300865
DOI:
10.1016/j.syapm.2011.12.001
[Indexed for MEDLINE]

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