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Nanomedicine (Lond). 2012 Apr;7(4):475-91. doi: 10.2217/nnm.11.112. Epub 2012 Feb 2.

Control of solid tumor growth in mice using EGF receptor-targeted RNA replicase-based plasmid DNA.

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1
College of Pharmacy, The University of Texas at Austin, Austin, TX 78712, USA.

Abstract

AIM:

Previously, it was shown that treatment of tumor-bearing mice with an RNA replicase-based plasmid that produces dsRNA when transfected into tumor cells significantly inhibited the tumor growth. In the present study, the feasibility of further improving the anti-tumor activity of the RNA replicase-based plasmid by targeting it into tumors cells was evaluated.

MATERIAL & METHODS:

An EGF-conjugated, polyethylene glycosylated cationic liposome was developed to deliver the RNA replicase-based plasmid, pSIN-β, into EGF receptor-overexpressing human breast cancer cells (MDA-MB-468) in vitro and in vivo.

RESULTS:

Delivery of pSIN-β using the EGF receptor-targeted liposome more effectively controlled the growth of MDA-MB-468 tumors (and human epidermoid carcinoma A431 tumors) in mice than using untargeted liposome. The pSIN-β carried by the EGF receptor-targeted liposome caused the complete regression of MDA-MB-468 tumors in mice, probably due to the enhancement of its proapoptotic, antiproliferative and antiangiogenic activities.

DISCUSSION:

Tumor-targeted RNA replicase-based plasmids hold a strong potential in tumor therapy.

PMID:
22296186
PMCID:
PMC3523886
DOI:
10.2217/nnm.11.112
[Indexed for MEDLINE]
Free PMC Article
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