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Sensors (Basel). 2010;10(3):2045-53. doi: 10.3390/s100302045. Epub 2010 Mar 12.

Signal amplification by enzymatic reaction in an immunosensor based on localized surface plasmon resonance (LSPR).

Author information

1
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. t2taehan@gmail.com

Abstract

An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-γ (IFN-γ) capture antibody was immobilized on the gold NIs, followed by binding of IFN-γ to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecular binding.

KEYWORDS:

enzyme-catalyzed precipitation; gold nano-island; immunosensor; localized surface plasmon resonance (LSPR)

PMID:
22294913
PMCID:
PMC3264466
DOI:
10.3390/s100302045
[Indexed for MEDLINE]
Free PMC Article
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