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Methods. 2012 Jul;57(3):318-30. doi: 10.1016/j.ymeth.2012.01.003. Epub 2012 Jan 24.

Flow cytometry of fluorescent proteins.

Author information

1
Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. telfordw@mail.nih.gov

Abstract

Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.

PMID:
22293036
DOI:
10.1016/j.ymeth.2012.01.003
[Indexed for MEDLINE]

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