Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Enzymol. 2012;505:445-68. doi: 10.1016/B978-0-12-388448-0.00031-0.

Illuminating mobile zinc with fluorescence from cuvettes to live cells and tissues.

Author information

1
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Abstract

With the aid of chemoselective sensors, fluorescence microscopy has emerged as an indispensable tool to visualize the distribution and dynamics of various biologically important molecules in live specimens. Motivated by our interest in understanding the chemistry and biology of mobile zinc underlying its physiological and pathological roles, over the past decade, our laboratory has developed an extensive library of zinc fluorescence probes. In this chapter, we provide essential information about our sensor toolbox in order to assist investigators interested to apply our constructs to study various aspects of mobile zinc biology. We illustrate their use with several examples of imaging both exogenous and endogenous mobile zinc in live cells and tissues using various versions of fluorescence microscopy, including confocal and two-photon microscopy.

[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center