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Exp Cell Res. 2012 Mar 10;318(5):662-9. doi: 10.1016/j.yexcr.2012.01.009. Epub 2012 Jan 18.

Megakaryocyte development is normal in mice with targeted disruption of Tescalcin.

Author information

1
Division of Pediatric Endocrinology, University of Miami, Miller School of Medicine, 1601 NW 12th Avenue, Miami, FL 33136, USA. sukarapong@yahoo.com

Abstract

BACKGROUND:

Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H+ exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and human CD34+ hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation.

OBJECTIVE:

To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis.

METHODS:

We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development.

RESULTS:

Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal.

CONCLUSION:

Tescalcin does not appear to be necessary for normal megakaryocyte development.

PMID:
22285131
DOI:
10.1016/j.yexcr.2012.01.009
[Indexed for MEDLINE]

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