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Genet Test Mol Biomarkers. 2012 May;16(5):406-11. doi: 10.1089/gtmb.2011.0166. Epub 2012 Jan 27.

High-resolution melting analyses for gene scanning of APC, MLH1, MSH2, and MSH6 associated with hereditary colorectal cancer.

Author information

1
Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan. jurat0922@yahoo.co.jp

Abstract

BACKGROUND:

Hereditary colorectal cancer accounts for approximately 4-5% of all colorectal cancers. The causative genes for familial adenomatous polyposis and hereditary nonpolyposis colorectal cancer are large, making comprehensive analyses difficult. Therefore, high-throughput and practical methods are required to make an early diagnosis of hereditary colorectal cancers and identify high-risk individuals. For this purpose, we developed a novel gene scanning method by high-resolution melting (HRM) analysis.

METHODS:

High-resolution melting (HRM) analysis is a promising prescreening method for nucleic acid sequence variants because of its high sensitivity and high-throughput capability. We evaluated HRM for screening APC, MLH1, MSH2, and MSH6 genes for point mutations, small deletions, and insertions. Simultaneously, we evaluated quantitative polymerase chain reaction-HRM (qPCR-HRM) for screening the MSH2 gene for large rearrangements.

RESULTS:

All 28 point mutations and 1 large rearrangement were successfully detected by qPCR-HRM analysis.

CONCLUSIONS:

A fast and reliable mutation detection strategy with HRM and qPCR-HRM was used to diagnose hereditary colorectal cancers. Because this method is simple and economical, it may be useful in diagnostic laboratories.

PMID:
22283331
DOI:
10.1089/gtmb.2011.0166
[Indexed for MEDLINE]

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