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Science. 2012 Feb 17;335(6070):856-9. doi: 10.1126/science.1209678. Epub 2012 Jan 26.

Epithelial nitration by a peroxidase/NOX5 system mediates mosquito antiplasmodial immunity.

Author information

1
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Rockville, MD 20892, USA.

Abstract

Plasmodium ookinetes traverse midgut epithelial cells before they encounter the complement system in the mosquito hemolymph. We identified a heme peroxidase (HPX2) and NADPH oxidase 5 (NOX5) as critical mediators of midgut epithelial nitration and antiplasmodial immunity that enhance nitric oxide toxicity in Anopheles gambiae. We show that the two immune mechanisms that target ookinetes-epithelial nitration and thioester-containing protein 1 (TEP1)-mediated lysis-work sequentially, and we propose that epithelial nitration works as an opsonization-like system that promotes activation of the mosquito complement cascade.

PMID:
22282475
PMCID:
PMC3444286
DOI:
10.1126/science.1209678
[Indexed for MEDLINE]
Free PMC Article

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