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Int J Mol Sci. 2011;12(12):9155-71. doi: 10.3390/ijms12129155. Epub 2011 Dec 8.

RNA aptamer evolution: two decades of SELEction.

Author information

1
Unit of Research on Oncological Disease, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico. guillaqui@himfg.edu.mx

Abstract

Aptamers are small non-coding RNAs capable of recognizing, with high specificity and affinity, a wide variety of molecules in a manner that resembles antibodies. This class of nucleic acids is the resulting product of applying a well-established screening method known as SELEX. First developed in 1990, the SELEX process has become a powerful tool to select structured oligonucleotides for the recognition of targets, starting with small molecules, going through protein complexes until whole cells. SELEX has also evolved along with new technologies positioning itself as an alternative in the design of a new class of therapeutic agents in modern molecular medicine. This review is an historical follow-up of SELEX method over the two decades since its first appearance.

KEYWORDS:

SELEX; aptamers; in vitro evolution; in vitro selection; non-coding RNAs

PMID:
22272125
PMCID:
PMC3257122
DOI:
10.3390/ijms12129155
[Indexed for MEDLINE]
Free PMC Article

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