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Obstet Gynecol. 2012 Feb;119(2 Pt 1):338-43. doi: 10.1097/AOG.0b013e318242a11d.

Preimplantation genetic diagnosis for fetal neonatal alloimmune thrombocytopenia due to antihuman platelet antigen maternal antibodies.

Author information

1
Medical Genetics Institute, ZOHAR Preimplantation Genetic Diagnosis Unit, Department of Obstetrics and Gynecology, Shaare Zedek Medical Center, Hebrew University Medical School, Jerusalem, Israel. gheona@szmc.org.il

Erratum in

  • Obstet Gynecol. 2012 Apr;119(4):872. Geva, T Eldar [corrected to Eldar-Geva, T].

Abstract

OBJECTIVE:

To develop a reliable preimplantation genetic diagnosis protocol for antihuman platelet antigen-1 incompatibility for a family in whom antenatal treatment was not possible because of the mother's hypersensitivity to intravenous immunoglobulin (IVIG).

METHODS:

Haplotypes were constructed from genomic DNA of the family members. A polymerase chain reaction protocol that included eight microsatellite polymorphic markers and the ITGB3-specific (T196C, rs5918) polymorphism were multiplexed to be used in a single cell protocol, and single blastomeres were analyzed.

RESULTS:

In one preimplantation genetic diagnosis cycle, out of 28 retrieved oocytes, 24 embryos fertilized and 12 underwent biopsy. Three embryos were found to be antihuman platelet antigen-1b/1b homozygotes and two were transferred. This cycle resulted in an uneventful pregnancy and birth of a healthy child.

CONCLUSION:

In cases in which there is antihuman platelet antigen incompatibility and IVIG cannot be administered, preimplantation genetic diagnosis is a reliable alternative to enable birth of unaffected children.

PMID:
22270286
DOI:
10.1097/AOG.0b013e318242a11d
[Indexed for MEDLINE]

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