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J Appl Microbiol. 2012 Apr;112(4):831-40. doi: 10.1111/j.1365-2672.2012.05241.x. Epub 2012 Feb 13.

Nisin F-loaded brushite bone cement prevented the growth of Staphylococcus aureus in vivo.

Author information

1
Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa.

Abstract

AIMS:

To determine if nisin F-loaded self-setting brushite cement could control the growth of Staphylococcus aureus in vivo.

METHODS AND RESULTS:

  Brushite cement was prepared by mixing equimolar concentrations of β-tricalcium phosphate and monocalcium phosphate monohydrate. Nisin F was added at 5·0, 2·5 and 1·0% (w/w) and the cement moulded into cylinders. In vitro antibacterial activity was determined using a delayed agar diffusion assay. Release of nisin F from the cement was determined using BCA protein assays. Based on scanning electron microscopy and X-ray diffraction analysis, nisin F did not cause significant changes in cement structure or chemistry. Cement containing 5·0% (w/w) nisin F yielded the most promising in vitro results. Nisin F-loaded cement was implanted into a subcutaneous pocket on the back of mice and then infected with S. aureus Xen 36. Infection was monitored for 7 days, using an in vivo imaging system. Nisin F prevented S. aureus infection for 7 days and no viable cells were isolated from the implants.

CONCLUSIONS:

Nisin F-loaded brushite cement successfully prevented in vivo growth of S. aureus.

SIGNIFICANCE AND IMPACT OF THE STUDY:

Nisin F incorporated into bone cement may be used to control S. aureus infection in vivo.

[Indexed for MEDLINE]
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