Format

Send to

Choose Destination
J Vet Intern Med. 2012 Mar-Apr;26(2):412-4. doi: 10.1111/j.1939-1676.2011.00882.x. Epub 2012 Jan 23.

Comparison of IV and IM formulations of synthetic ACTH for ACTH stimulation tests in healthy dogs.

Author information

1
Animal Surgical and Emergency Center, 1535 S. Sepulveda Boulevard, Los Angeles, CA 90025, USA. toddcohen21@gmail.com

Abstract

BACKGROUND:

Two commercially available forms of synthetic ACTH are used to diagnose and monitor hyper- and hypoadrenocorticism in dogs.

OBJECTIVE:

To compare the biologic activity of the liquid and lyophilized forms of cosyntropin.

ANIMALS:

Eighteen privately owned healthy dogs were included.

METHODS:

Dogs were assigned to one of 2 groups of 9 dogs each. Group 1 dogs were tested with the lyophilized product first and the liquid solution 30-60 days later. The Group 2 dogs were tested with the liquid solution first and the lyophilized drug 30-60 days later. For the ACTH stimulation tests, serum samples were collected before and 1 hour after IM administration of 0.25 mg reconstituted lyophilized product or 1 hour after IV administration of 0.25 mg of liquid solution. Cortisol concentrations of all serum samples were measured by use of a commercial cortisol radioimmunoassay.

RESULTS:

Serum cortisol concentrations before and after ACTH stimulation did not differ significantly between groups (P = .57). In addition, no individual dog had as much as a 20% difference in serum cortisol concentrations after administration of either ACTH formulation.

CONCLUSIONS AND CLINICAL IMPORTANCE:

Given the lack of significant differences of the ACTH stimulation test results, the lyophilized and liquid solution products can be used interchangeably.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center