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Int J Nanomedicine. 2011;6:3393-404. doi: 10.2147/IJN.S27830. Epub 2011 Dec 20.

Comparative manufacture and cell-based delivery of antiretroviral nanoformulations.

Author information

1
Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.

Abstract

Nanoformulations of crystalline indinavir, ritonavir, atazanavir, and efavirenz were manufactured by wet milling, homogenization or sonication with a variety of excipients. The chemical, biological, immune, virological, and toxicological properties of these formulations were compared using an established monocyte-derived macrophage scoring indicator system. Measurements of drug uptake, retention, release, and antiretroviral activity demonstrated differences amongst preparation methods. Interestingly, for drug cell targeting and antiretroviral responses the most significant difference among the particles was the drug itself. We posit that the choice of drug and formulation composition may ultimately affect clinical utility.

KEYWORDS:

human immunodeficiency virus type one; manufacturing techniques; monocyte-derived macrophage; nanoformulated antiretroviral therapy; nanotoxicology

PMID:
22267924
PMCID:
PMC3260033
DOI:
10.2147/IJN.S27830
[Indexed for MEDLINE]
Free PMC Article

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