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Appl Environ Microbiol. 2012 Apr;78(7):2470-3. doi: 10.1128/AEM.06527-11. Epub 2012 Jan 20.

Use of amplified-fragment length polymorphism to study the ecology of Campylobacter jejuni in environmental water and to predict multilocus sequence typing clonal complexes.

Author information

1
Department of Microbiology and Infectious Diseases, Faculté de Médecine de l’Université de Sherbrooke, Québec, Canada.

Abstract

We determined the genetic variability among water isolates of Campylobacter jejuni by using amplified-fragment length polymorphism (AFLP) and multilocus sequence typing (MLST). Across a highly diverse collection of isolates, AFLP clusters did not correlate with MLST clonal complexes, suggesting that AFLP is not reliable for deciphering population genetic relationships and may be problematic for larger epidemiologic analyses.

PMID:
22267674
PMCID:
PMC3302600
DOI:
10.1128/AEM.06527-11
[Indexed for MEDLINE]
Free PMC Article
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