Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2012;844:183-7. doi: 10.1007/978-1-61779-527-5_13.

Isolation and differentiation of monocytes-macrophages from human blood.

Author information

1
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, Australia.

Abstract

The prevalence of fungal infections remains high, and it is associated with significant mortality and morbidity. Macrophages are heterogeneous population of effectors enriched in regions of Candida colonization. These cells sense Candida, and are critical in the resolution of these infections. Here, we describe how macrophages are generated in the presence of colony-stimulating factor-1 (CSF-1); an important cytokine required for the survival, proliferation and ex-vivo differentiation of monocytes to macrophages.

PMID:
22262443
DOI:
10.1007/978-1-61779-527-5_13
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center