Format

Send to

Choose Destination
See comment in PubMed Commons below
J Mol Biol. 2012 Feb 24;416(3):389-99. doi: 10.1016/j.jmb.2012.01.002. Epub 2012 Jan 10.

Temporal regulation of gene expression of the Escherichia coli bacteriophage phiEco32.

Author information

1
Waksman Institute for Microbiology, Piscataway, NJ 08854, USA.

Abstract

Escherichia coli phage phiEco32 encodes two proteins that bind to host RNA polymerase (RNAP): gp79, a novel protein, and gp36, a distant homolog of σ(70) family proteins. Here, we investigated the temporal pattern of phiEco32 and host gene expression during infection. Host transcription shutoff and three distinct bacteriophage temporal gene classes (early, middle, and late) were revealed. A combination of bioinformatic and biochemical approaches allowed identification of phage promoters recognized by a host RNAP holoenzyme containing the σ(70) factor. These promoters are located upstream of early phage genes. A combination of macroarray data, primer extension, and in vitro transcription analyses allowed identification of six promoters recognized by an RNAP holoenzyme containing gp36. These promoters are characterized by a single-consensus element tAATGTAtA and are located upstream of the middle and late phage genes. Curiously, gp79, an inhibitor of host and early phage transcription by σ(70) holoenzyme, activated transcription by the gp36 holoenzyme in vitro.

PMID:
22261232
PMCID:
PMC3275717
DOI:
10.1016/j.jmb.2012.01.002
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center